The requirements for performing IUI and IVF when it comes to yield, and percentage of motile
sperm can differ.
Optimize the results by using a 40/80 gradient when a maximum yield is wanted for IUI and
40/90 for the highest percentage of motile sperm for IVF or ICSI.
Bubbles seen in IVF media after transport do not generally affect the quality. They are often a result from the presence of human serum albumin (hSA)- a common additive in culture media, like in PureSperm Wash. HSA contains both hydrophilic and hydrophobic regions....
Two types of high quality sperm separations Nidacons product SpeediKit and a regular density gradient both serve the purpose of creating high quality sperm separations however, there are some distinct advantages to SpeediKit over a traditional density gradient. Key...
All solutions at room temperature Prepare the gradient using 2 ml layers. Prepare the gradient no more than 30 minutes before use to prevent the layers from mixing. Avoid overloading—do not exceed the recommended 1.5 ml of sample Follow the recommended centrifugation...
Refrigerated Incubation (10–60 minutes): Incubating sperm in the refrigerator prior to time on liquid nitrogen for 10 to 60 minutes improves survival rates. While the difference between 10 and 60 minutes is slight, there is a significant increase in survival when...
Additional information regarding Mouse Embryo Assay (MEA) results on day 6 will be provided on the Quality Assurance certificates for Nidoil. The Day 6 (eMEA) results offer an extra layer of safety assessment, enhancing our ability to detect potential toxicity and...
Semen osmolality increases during and after liquefaction due to enzymatic degradation of molecules in semen. The increased osmolality in the ejaculate can negatively impact the outcome of sperm preparation. To minimize this risk, early dilution of the ejaculate is...
Can a washing solution help you to optimize your result? When comparing PureSpermWash to other washing buffers, it has been shown that PSW • maintains the correct ROS production better (1) • achieves the highest percentage of capacitated sperm (1) • produces higher...
Would you like to elevate your expertise in handling sperm samples? Our comprehensive semen workshop covers everything you need to know about sperm preparation, including optimizing the process and mastering sperm freezing techniques. While we haven't finalized the...
Concerns may arise regarding the effect of extreme weather conditions on product performance. To address these concerns, all Nidacon products undergo rigorous testing for elevated temperatures up to 50°C and down to -18°C over a span of 5 days. The results of these...
Sperm are highly sensitive to temperature fluctuations during storage, it can have a negative effect on both semen motility and DNA integrity. The best way to store the sample is at room temperature (RT). Compared to storage at 37°C, maintaining samples at RT...