Sperm are highly sensitive to temperature fluctuations during storage, it can have a negative effect on both semen motility and DNA integrity.
The best way to store the sample is at room temperature (RT). Compared to storage at 37°C, maintaining samples at RT significantly reduces DNA fragmentation, ensuring a superior and more enduring preservation of sperm quality during incubation.
Ref. Influence of temperature and sperm preparation on the quality of spermatozoa
Thijssen A et al Biomed Online 2014