Semen osmolality increases during and after liquefaction due to enzymatic degradation of molecules in semen.
The increased osmolality in the ejaculate can negatively impact the outcome of sperm preparation. To minimize this risk, early dilution of the ejaculate is recommended. PureSpermBuffer or PureSpermWash are ideal buffers for this purpose. For best results, dilute the ejaculate 1:1 in the tube as soon as possible.
Reference: Holmes, Björndahl, L., and Kvist, U. (2019). Possible factors influencing post‐ejaculatory changes of the osmolality of human semen in vitro. Andrologia, 51(11), e13443–n/a. https://doi.org/10.1111/and.13443