Flexible freezing protocol

Feb 2021 | Tips & Tricks

Exact time protocols to follow, patients arriving at a specific time, etc., can sometimes be quite stressful. We, therefore, have a more flexible protocol for our recommended freezing method for sperm using SpermCryoProtec.

Two flexible variables

  1. Incubation time in the fridge after loading the straws.

The range is 10-60 minutes. Any difference between 10 and 60 minutes? The survival results, when incubating for 60 minutes compared to 10, shows a slight increase when incubated for 60 minutes. The major difference however is, between not incubating and incubating for 10 minutes.

  1. Time on the CryoFloater in LN2

The recommended time is 10-30 minutes. Measuring the temperature on the floater, it goes down quite fast and 10 minutes is more than enough but, in order to make it more flexible, we recommend the range of 10-30. No difference in result with regards to survival rate has been found in our tests between 10 and 30 minutes but it will give you the chance to take the coffee you so desperately need!

Other Tips & Tricks

Nidoil – Improved Quality Assurance

Sep 2024

Additional information regarding Mouse Embryo Assay (MEA) results on day 6 will be provided on the Quality Assurance certificates for Nidoil. The Day 6 (eMEA) results offer an extra layer of safety assessment, enhancing our ability to detect potential toxicity and...

Optimize your sperm preparation

Aug 2024

Semen osmolality increases during and after liquefaction due to enzymatic degradation of molecules in semen. The increased osmolality in the ejaculate can negatively impact the outcome of sperm preparation. To minimize this risk, early dilution of the ejaculate is...

Optimize your results

May 2024

Can a washing solution help you to optimize your result? When comparing PureSpermWash to other washing buffers, it has been shown that PSW • maintains the correct ROS production better (1) • achieves the highest percentage of capacitated sperm (1) • produces higher...

Enhance your sperm preparation skills

Apr 2024

Would you like to elevate your expertise in handling sperm samples? Our comprehensive semen workshop covers everything you need to know about sperm preparation, including optimizing the process and mastering sperm freezing techniques. While we haven't finalized the...

Is transportation without cold chain safe?

Jan 2024

Concerns may arise regarding the effect of extreme weather conditions on product performance. To address these concerns, all Nidacon products undergo rigorous testing for elevated temperatures up to 50°C and down to -18°C over a span of 5 days. The results of these...

DNA fragmentation due to storage

Nov 2023

Sperm are highly sensitive to temperature fluctuations during storage, it can have a negative effect on both semen motility and DNA integrity. The best way to store the sample is at room temperature (RT). Compared to storage at 37°C, maintaining samples at RT...

Handling of Cryofloater

Oct 2023

  Utilizing the Cryofloater facilitates a simple procedure for sperm cryopreservation, resulting in an elevated survival rate. The cryofloater can be used for an extended period, provided that it is handled with care post-procedure due to it being brittle when...

Shelf life after opening

Sep 2023

Reminder, the shelf life of all our products remains unchanged even after opening. By storing them in a cool environment and handling them with care, you can ensure they maintain their high-quality performance throughout their entire shelf life. The only exception to...

Viscous Semen Sample

Apr 2023

Obtaining satisfactory sperm yield from highly viscous semen samples remains a major problem for laboratories providing Tips and Tricks: Viscous samples can be treated with PureSperm® Buffer. You simply add PureSperm®Buffer to the ejaculate (dilution 1+3), 1 part...

Make the perfect Gradient

Mar 2023

Slowly layering gradients with visible rafts between the layers, no more than 60 minutes before use ensures maximum sharpness. The rafts take up a lot of the unwanted components of the ejaculate like dead spermatozoa, other cells etc and the gradient will provide a...