Refrigerated Incubation (10–60 minutes): Incubating sperm in the refrigerator prior to time on liquid nitrogen for 10 to 60 minutes improves survival rates. While the difference between 10 and 60 minutes is slight, there is a significant increase in survival when comparing 10 minutes of incubation to none at all.
Time on liquid nitrogen (10–30 minutes): This step involves placing the samples on a floater (Nidacon CrypFloater) for 10 to 30 minutes. Survival rates remain consistent throughout this period, so the choice depends on your workflow. If opting for the full 30 minutes, ensure that sufficient liquid nitrogen (LN2) is available to maintain optimal conditions.
The requirements for performing IUI and IVF when it comes to yield, and percentage of motile sperm can differ. Optimize the results by using a 40/80 gradient when a maximum yield is wanted for IUI and 40/90 for the highest percentage of motile sperm for IVF or ICSI.
Additional information regarding Mouse Embryo Assay (MEA) results on day 6 will be provided on the Quality Assurance certificates for Nidoil. The Day 6 (eMEA) results offer an extra layer of safety assessment, enhancing our ability to detect potential toxicity and...
Semen osmolality increases during and after liquefaction due to enzymatic degradation of molecules in semen. The increased osmolality in the ejaculate can negatively impact the outcome of sperm preparation. To minimize this risk, early dilution of the ejaculate is...
Can a washing solution help you to optimize your result? When comparing PureSpermWash to other washing buffers, it has been shown that PSW • maintains the correct ROS production better (1) • achieves the highest percentage of capacitated sperm (1) • produces higher...
Would you like to elevate your expertise in handling sperm samples? Our comprehensive semen workshop covers everything you need to know about sperm preparation, including optimizing the process and mastering sperm freezing techniques. While we haven't finalized the...
Concerns may arise regarding the effect of extreme weather conditions on product performance. To address these concerns, all Nidacon products undergo rigorous testing for elevated temperatures up to 50°C and down to -18°C over a span of 5 days. The results of these...
Sperm are highly sensitive to temperature fluctuations during storage, it can have a negative effect on both semen motility and DNA integrity. The best way to store the sample is at room temperature (RT). Compared to storage at 37°C, maintaining samples at RT...
Utilizing the Cryofloater facilitates a simple procedure for sperm cryopreservation, resulting in an elevated survival rate. The cryofloater can be used for an extended period, provided that it is handled with care post-procedure due to it being brittle when...
Reminder, the shelf life of all our products remains unchanged even after opening. By storing them in a cool environment and handling them with care, you can ensure they maintain their high-quality performance throughout their entire shelf life. The only exception to...
Obtaining satisfactory sperm yield from highly viscous semen samples remains a major problem for laboratories providing Tips and Tricks: Viscous samples can be treated with PureSperm® Buffer. You simply add PureSperm®Buffer to the ejaculate (dilution 1+3), 1 part...
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