by Oscar Rymo | Feb 19, 2025 | News, Tips & Tricks
All solutions at room temperature Prepare the gradient using 2 ml layers. Prepare the gradient no more than 30 minutes before use to prevent the layers from mixing. Avoid overloading—do not exceed the recommended 1.5 ml of sample Follow the recommended centrifugation...
by Anders | Jan 24, 2025 | Tips & Tricks
The requirements for performing IUI and IVF when it comes to yield, and percentage of motile sperm can differ. Optimize the results by using a 40/80 gradient when a maximum yield is wanted for IUI and 40/90 for the highest percentage of motile sperm for IVF or...
by Anders | Nov 24, 2024 | Tips & Tricks
Refrigerated Incubation (10–60 minutes): Incubating sperm in the refrigerator prior to time on liquid nitrogen for 10 to 60 minutes improves survival rates. While the difference between 10 and 60 minutes is slight, there is a significant increase in survival when...
by Oscar Rymo | Sep 26, 2024 | News, Tips & Tricks
Additional information regarding Mouse Embryo Assay (MEA) results on day 6 will be provided on the Quality Assurance certificates for Nidoil. The Day 6 (eMEA) results offer an extra layer of safety assessment, enhancing our ability to detect potential toxicity and...
by Oscar Rymo | Aug 26, 2024 | Tips & Tricks
Semen osmolality increases during and after liquefaction due to enzymatic degradation of molecules in semen. The increased osmolality in the ejaculate can negatively impact the outcome of sperm preparation. To minimize this risk, early dilution of the ejaculate is...
by Oscar Rymo | May 26, 2024 | Tips & Tricks
Can a washing solution help you to optimize your result? When comparing PureSpermWash to other washing buffers, it has been shown that PSW • maintains the correct ROS production better (1) • achieves the highest percentage of capacitated sperm (1) • produces higher...
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