Obtaining satisfactory sperm yield from highly viscous semen samples remains a major problem for laboratories providing
Tips and Tricks:
Viscous samples can be treated with PureSperm® Buffer.
You simply add PureSperm®Buffer to the ejaculate (dilution 1+3), 1 part PureSperm®Buffer and 3 parts sample (e.g. 0.5 ml PureSperm® Buffer + 1.5 ml semen sample).
Incubate for 15-30 minutes at 37°C, mix using a pipette and the sample is ready for preparation (preferably using a density gradient).
Kind regards,
Nidacon Team