ThermoBlast Kit 4x10mL
Based on clinically tested, well established, standard formulations.
(Not available in Europe)
Nidacon’s ThermoBlast media is based on well-tested standard formulations (Lane et al) and are both optimised for the thawing of blastocysts. Since these products have been used in practice for a considerable time and thoroughly tested, Nidacon can provide detailed protocols for vitrifying blastocysts, ensuring the most reliable and secure process possible.
Features
Clinical performance benefits:
- High blastocyst survival rate.
- Good implantations rates when used for ART techniques.
Specific claims for ThermoBlast:
- A pH stable formulation, both in room temperature and when incubated in 5-6% CO2.
- Long shelf life of one year and storage in room temperature.
Components
- Sodium chloride
- Potassium chloride
- Magnesium sulphate
- Potassium dihydrogen phosphate
- Sodium bicarbonate
- hSA (human Serum Albumin)
- Purified water
- Glucose
- Calcium lactate
- Sodium pyruvate
- EDTA
- HEPES
- Sucrose
Performance Characteristics
- pH: 7.20-7.50
- Endotoxin levels: <0.5 EU/mL.
- MEA: Re-expanded blastocysts after exposure >80%.
- Bottles and screw caps are M.E.A. tested.
Storage and Stability
- Store between 2 and 30°C and avoid temperatures above or below these values. VitriBlast has a shelf-life of 12 months from date of production when stored under these conditions. The expiry date is shown on both bottles and cartons.
- Open and close bottles under sterile conditions. After opening, store between 2 and 8°C when not in use. The shelf-life given on the product label applies only when the product is stored according to manufacturer’s recommendations.
- No antibiotics, unstable additives, or preservatives have been added to VitriBlast or ThermoBlast by the manufacturer.
Precautions and warnings
- Use aseptic procedures at all times.
- Do not use any vial or solution that appears cloudy or shows evidence of particulate matter.
- VitriBlast contains DMSO which is a highly penetrative substance. A material safety data sheet is available from the distributor or manufacturer (see nidacon.com).
- VitriBlast contains Ethylene glycol which is toxic when ingested.
- A Closed Vitrification system is mandatory in USA.
- Federal Law (USA) restricts this device to sale by or on the order of a physician.
- Please check for regulatory compliance governing the use of ART products in your country.
General recommendations
- Do not aspirate solution from TB5 before aspirating from TB4. This is to allow the blastocyst to sink in TB5 which makes it easier to identify. Do not aspirate solution from TB6 when aspirating from TB5. This is to allow the blastocyst to sink in TB6 which again makes it easier to identify.
Documents
References
Prediction of live birth in frozen-thawed single blastocyst transfer cycles by pre-freeze and post-thaw morphology.
Ahlström A1, Westin C, Wikland M, Hardarson T., (2013)
Human Reproduction, Vol.28, No.5 pp. 1199–1209
Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique.
Lane M et al, (1999)
Fertility and Sterility., Vol 72, No 6, pp1073-1078
Vitrification of human blastocysts using cryoloops: Clinical outcome of 223 cycles.
Mukaida T et al. (2003)
Hum Reprod., Vol. 18, No. 2, pp384-391
Perinatal outcome of blastocyst transfer with vitrification using cryoloop: A 4 year follow-up study.
Takahashi K et al. (2005)
Fertil Steril., Vol. 84, No. 1, 88-92
Obstetric outcome of children born after transfer of vitrified blastocysts
Wikland M et al. (2010).
Human Reproduction, Vol.00, No.0 pp. 1–9
Vitrification and warming human blastocysts by use of a laser to artificially induce blastocyst collapse prior to vitrification.
T. Hardarson et al. (2006)
Acta Obstet Gynecol Scand. 86 pp119-120