Sperm VitalStain
Optimised one step staining technique for assessment of sperm vitality.
One of the basic tools used in semen analysis, sperm vitality assessment is a key component of successful IVF. Sperm VitalStain, which contains both eosin and nigrosine, is a key component of Nidacon’s optimised sperm vitality assessment staining technique. Our technique is based on the principle that dead cells (i.e., those with a damaged plasma membrane) will absorb the eosin and exhibit a red stain. The nigrosine is used as a counterstain to facilitate visualization of the unstained (white) live cells.
Features
SpermVitalStain uses the eosin-nigrosine technique in a one-step method to establish the percentage of live spermatozoa. It is based on the principle that dead cells (i.e. those with damaged plasma membranes) will take up the eosin and stain red. Nigrosine provides the background to facilitate visualisation of the unstained (white) live cells.
Components
- Sodium chloride
- Eosin Y
- Purified water
- Nigrosine
- Formalin
Storage and Stability
Store between 10-40°C. When stored under these conditions, Sperm VitalStain has a shelf-life of 24 months from date of production. Expiry date is shown on both bottles and cartons.
Precautions and warnings
- Sperm VitalStain is non-flammable and does not present a fire or combustion hazard. A material safety data sheet is available upon request.
- Do not use contents if the tamper-evident seal is broken.
- If Sperm VitalStain comes in contact with skin, rinse thoroughly with water.
- If Sperm VitalStain comes in contact with eyes, rinse thoroughly with water for 15 minutes and then contact the nearest hospital.
General recommendations
Stained slides can be stored, preferably mounted and kept in darkness.
References
Why the WHO Recommendations for Eosin-Nigrosin Staining Techniques for Human Sperm Vitality Assessment Must Change
Lars Björndahl, Inget Söserlund, Sofia Johansson, Majid Mohammadieh, Mohammad Reza Pourian and Ulrik Kvist.
Journal of Andrology, Vol. 25, No. 5, September/October 2004
FAQ
How many cells do I need to count?
We recommend that you count 200 sperm to get an accurate classification. The 100x objective with immersion oil will give you a very clear picture of the stained versus unstained sperm cells.
If the sperm is only coloured at the neck is classifies as dead or alive?
It’s classified as alive.