Sperm VitalStain 2 x 20 mL

Sperm VitalStain 2 x 20 mL

Cat. No. SVS-010 Category:

Optimised one step staining technique for assessment of sperm vitality.

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Sperm vitality assessment is one of the basic tools used in semen analysis. Sperm Vitality should be determined in semen samples with 50% or more immotile spermatozoa according to WHO laboratory manual for examination of human sperm.

Features

SpermVitalStain uses the eosin-nigrosine technique in a one-step method to establish the percentage of live spermatozoa. It is based on the principle that dead cells (i.e. those with damaged plasma membranes) will take up the eosin and stain red. Nigrosine provides the background to facilitate visualisation of the unstained (white) live cells.

Components
  • Sodium chloride
  • Eosin Y
  • Purified water
  • Nigrosine
  • Formalin
Performance characteristics
  • pH 7.3 – 9.5
  • Functional analysis

 

Storage and Stability

  • Store the product at a temperature between 10 and 40°C. Avoid storing it at temperatures below 10°C or above 40°C.

  • When stored unopened within this temperature range, Sperm VitalStain has a shelf life of 12 months from the date of production. The expiry date is printed on both the bottles and the cartons.

  • Once a bottle has been opened, the product should be used within 6 months.

  • It is important to keep the product unopened and handle it according to these storage guidelines to ensure its optimal performance and longevity.

Precautions and warnings
  • Sperm VitalStain is non-flammable and does not present a fire or combustion hazard. A material safety data sheet is available upon request.
  • Do not use contents if the tamper-evident seal is broken.
  • If Sperm VitalStain comes in contact with skin, rinse thoroughly with water.
  • If Sperm VitalStain comes in contact with eyes, rinse thoroughly with water for 15 minutes and then contact the nearest hospital.
General recommendations

  • Shake the bottle of Sperm VitalStain well before use to ensure proper mixing.

  • Stained slides can be stored, preferably mounted and kept in darkness. If they need to be preserved for later analysis.

  • The 100x objective with immersion oil will give a very clear picture of stained versus unstained sperm.
Documents

SDS – View PDF

Product insert (latest version) – View PDF

Leaflet – View PDF

References
Why the WHO Recommendations for Eosin-Nigrosin Staining Techniques for Human Sperm Vitality Assessment Must Change

Lars Björndahl, Inget Söserlund, Sofia Johansson, Majid Mohammadieh, Mohammad Reza Pourian and Ulrik Kvist.
Journal of Andrology, Vol. 25, No. 5, September/October 2004

FAQ
How many cells do I need to count?

We recommend that you count 200 sperm to get an accurate classification. The 100x objective with immersion oil will give you a very clear picture of the stained versus unstained sperm cells.

If the sperm is only coloured at the neck, is it classified as dead or alive?

It’s classified as alive.