PureSperm Wash – 2×20 ml

Cat. No. PSW-020 Category:

A three-in-one product.

PSW-020 2×20 mL (Not available in Europe)

PureSperm Wash has been optimised to serve various different purposes. This one product can be used for washing the sperm pellet recovered after density gradient preparation, for use in swim-up procedures, for extending the sperm prior to Intra Uterine Insemination (IUI), and as a maintenance medium.

Features
  • Thanks to its stable formulation, PureSperm Wash has a shelf-life up to 1 year.
  • PureSperm Wash does not contain any colouring agents or preservatives, both of which can be toxic to sperm.
  • PureSperm Wash is a sterile isotonic salt solution.
Components
  • Sodium chloride
  • Potassium chloride
  • Magnesium sulphate
  • Potassium dihydrogen phosphate
  • Sodium bicarbonate
  • Purified water
  • hSA (human Serum Albumin)
  • Glucose
  • Calcium lactate
  • Sodium pyruvate
  • EDTA
  • HEPES

Performance Characteristics

  • pH: 7.3-8.5.
  • Osmolality (mOsm/kg H2O): 290-300.
  • Endotoxin levels: <1.0 EU/mL.
  • Sperm survival 18 hours after density gradient separation: >70%.
  • Contents are tested by human sperm survival only.
  • Bottles and stoppers are M.E.A. tested.
Storage and Stability
  • Store unopened bottles between 2 and 30ºC. Avoid temperatures above or below these values. When stored under these conditions, Pure Sperm Wash has a shelf-life of 12 months from date of production. The expiry date is shown on both bottles and cartons.
  • Open and close bottles under aseptic conditions. After opening, store between 2 and 8ºC when not in use.
  • The shelf-life described on the product label is only applicable when the product is stored according to manufacturer’s recommendations.
  • No antibiotics, unstable additives, or preservatives have been added to PureSperm Wash.
Precautions and warnings
  • Use aseptic procedures at all times.
  • If available, use sealed centrifuge buckets during centrifugation to avoid creation of aerosols.
  • PureSperm Wash does not present a fire or combustion hazard. A material safety data sheet is available from the distributor or manufacturer.
  • Do not use any solution which shows evidence of bacterial contamination.
  • Do not use contents if tamper-evident seal is broken.
  • Federal Law (USA) restricts this device to sale by or on the order of a physician.
  • Please check for regulatory compliance governing the use of ART products in your country.
General recommendations
Documents

SDS – View PDF

Product insert (latest version) – View PDF

For other languages, contact Nidacon

References
PureSperm Wash Poster
The new and improved Wash poster
Evolution of the Effects of Different In Vitro Incubation Conditions on Sperm DNA Fragmentation

Forsell et al
Presented at Nordic Fertility Society meeting 2011 in Malmo, Sweden

Platelet-activating factor significantly enhances intrauterine insemination pregnancy rates in non-male factor infertility.

W. Roudebush, A. Toledo, H. Kort, D. Mitchell-Leef, C. Elsner, J. Massey
Fertility and Sterility, Volume 82, Issue 1, Pages 52-56

FAQ
When should PureSperm Wash be used?

We recommend PureSperm Wash for washing the sperm pellet after centrifugation using a PureSperm density gradient. The ionic balance of PureSperm Wash lies between that of the gradient and commonly used fertilisation media and, therefore, will not cause premature hyperactivation of the sperm. PureSperm Wash is also ready-to-use and contains an appropriate level of human serum albumin.

What are the constituents of PureSperm Wash?

PureSperm Wash is an isotonic (290-300 mOsm/kg H2O), HEPES-buffered salt solution containing glucose, EDTA, and human serum albumin.

Is there any protein in PureSperm Wash?

Yes. Research in our laboratory showed that human serum albumin must be added to the PureSperm Wash to prevent sperm sticking to plastic-ware and microscope slides.

Are antibiotics included in PureSperm Wash?

No, PureSperm Wash does not contain antibiotics. First of all, antibiotics commonly used in cell culture media are toxic to sperm and the gradient will remove most, if not all, of the bacterial contamination present in the ejaculate, provided that the retrieval of the sperm pellet is carried out according to the instructions given in the package insert.

Should PureSperm Wash be refrigerated?

PureSperm Wash is supplied as a sterile solution which does not need refrigerated storage in the unopened bottle. After opening (under sterile conditions), however, PureSperm Wash should be kept at 2-8°C.