PureSperm SpeediKit

PureSperm SpeediKit

Cat. No. PSSK-010 Category:

PureSperm Speedkit provides an alternative method of preparing human sperm for IUI – the UniLayer filtration of semen.

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The PureSperm SpeediKit offers a rapid and efficient sperm preparation method ideal for smaller clinics or IUI procedures. It simplifies the process by using a single-layer centrifugation technique, reducing preparation time compared to traditional multiple-layer density gradients. In addition a “swim-up” process does not remove sperm with abnormal morphology or damaged DNA, and does not seperate out bacteria and viruses from the sperm.
The kit contains all necessary components, including prefilled tubes of PureSperm 80 (Unilayer) and PureSperm Wash, allowing for convenient, ready-to-use procedures without the need for an incubator. The streamlined protocol ensures isolation of motile, viable spermatozoa while eliminating impurities, enabling quick preparation without compromising quality.

 

 

Features

  • Provides a rapid and efficient alternative to traditional density gradient sperm preparation using a single-layer centrifugation technique.

  • The kit contains all necessary components, including PureSperm colloid and PureSperm Wash, pre-dispensed in Type 1 glass centrifuge tubes for up to 10 patients.

  • Designed to isolate motile, viable sperm while removing dead sperm, abnormal morphology, cellular debris, bacteria, and viruses.

  • Helps remove toxic substances including reactive oxygen species (ROS) and decapacitation factors, improving sperm quality.

  • Recommended especially for smaller clinics or intrauterine insemination (IUI) procedures due to ease of use and speed.

Components
  • Sodium chloride
  • Potassium chloride
  • Magnesium sulphate
  • Potassium dihydrogen phosphate
  • Sodium bicarbonate
  • EDTA
  • hSA (human Serum Albumin)
  • Sodium citrate
  • Glucose
  • Calcium lactate
  • Sodium pyruvate
  • HEPES
  • Purified water
  • Silane-coated silica
Performance Characteristics
  • pH of PureSperm 80 (UniLayer): 7.4 – 7.8.
  • pH of PureSperm Wash: 7.3 – 8.5.
  • Osmolality of PureSperm UniLayer (mOsm/kg H2O): 300 – 330.
  • Osmolality of PureSperm Wash (mOsm/kg H2O): 290 – 300.
  • Endotoxin levels (EU/mL): <1.0.
  • Contents are tested by human sperm survival only.
  • Tubes and stoppers are M.E.A. tested.
Storage and Stability
  • Store between 2 and 30ºC. Avoid temperatures above or below these values. The kit has a shelf-life of 12 months from date of production. The expiry date is shown on both tubes and cartons.
  • The shelf-life described on the product label is only applicable when the product is stored according to manufacturer’s recommendations.
  • No antibiotics, unstable additives or preservatives have been added to PureSperm SpeediKit.
Precautions and warnings
  • When retrieving the sperm pellet, follow the instructions given in the pack insert to avoid inadvertent contamination.
  • Use aseptic procedures at all times.
  • If available, use sealed buckets during centrifugation to avoid creation of aerosols.
  • Clean accidental spills using a dampened cloth or paper. Spilled PureSperm SpeediKit causes floors and benches to be extremely slippery.
  • PureSperm SpeediKit does not present a fire or combustion hazard. A material safety data sheet is available from the distributor or manufacturer.
  • Do not use any solution which shows evidence of bacterial contamination.
  • Do not use contents if tamper-evident seal is broken.
  • Federal Law (USA) restricts this device to sale by or on the order of a physician.
  • Please check for regulatory compliance governing the use of ART products in your country.
General recommendations

  • The kit is designed for rapid and easy sperm preparation by centrifugation through a single layer of PureSperm 80 (unilayer) ideal for smaller clinics and intrauterine insemination (IUI) procedures.

  • All components needed for up to 10 patient preparations come pre-dispensed in Type 1 glass centrifuge tubes, including PureSperm 80 (unilayer) and PureSperm Wash, plus semen collection tubes.

  • The semen sample (up to 1.5 mL) should be carefully layered on top of the PureSperm 80 (unilayer).

  • Use sterile pipettes and aseptic techniques throughout the procedure to avoid contamination.

Documents

SDS-PSW – View PDF
SDS – PSUL – View PDF

Product insert – View PDF

For other languages, contact Nidacon

References
FAQ
What do I need to perform the sperm preparation using Speedikit?

You only need a centrifuge and some sterile pipettes. PureSperm Speedikit includes the prefilled tubes for both separation and washing of the sample.

Do I need an incubator for performing a sperm preparation with Speedikit?

No, the whole procedure is performed at room temperature.