PureSperm SpeediKit
PureSperm Speedkit provides an alternative method of preparing human sperm for IUI – the UniLayer filtration of semen.
PSSK-010 10 patient preps
Layering a density gradient correctly is difficult and achieving optimal results can be challenging. In addition, a “swim up” process does not remove sperm with abnormal morphology or damaged DNA, and does not separate out bacteria and viruses from the sperm preparation. For this reason, many clinicians in small clinics or private practices are looking for a better, safe, and effective method of preparing sperm for intra-uterine insemination that will improve their results.
PureSperm SpeediKit is a rapid and efficient alternative to sperm-preps using density gradient centrifugation. Everything is includedin a convenient kit form for quick sperm preparation, all based on the effective centrifugation through a single layer of PureSperm colloid, followed byrinsing the sperm with PureSperm Wash. The kit contains both the PureSperm colloid and the PureSperm Wash for 10 patients, already dispensed in centrifuge tubes. You do not need an incubator.
Features
The PureSperm SpeediKit is an alternative method of preparing human sperm for IUI. The kit contains sufficient sperm preparation materials for ten patients, already dispensed in Type 1 glass centrifuge tubes.
Components
- Sodium chloride
- Potassium chloride
- Magnesium sulphate
- Potassium dihydrogen phosphate
- Sodium bicarbonate
- EDTA
- hSA (human Serum Albumin)
- Sodium citrate
- Glucose
- Calcium lactate
- Sodium pyruvate
- HEPES
- Purified water
- Silane-coated silica
Performance Characteristics
- pH of PureSperm UniLayer: 7.4 – 7.8.
- pH of PureSperm Wash: 7.3 – 8.5.
- Osmolality of PureSperm UniLayer (mOsm/kg H2O): 300 – 330.
- Osmolality of PureSperm Wash (mOsm/kg H2O): 290 – 300.
- Endotoxin levels (EU/mL): <1.0.
- Contents are tested by human sperm survival only.
- Tubes and stoppers are M.E.A. tested.
Storage and Stability
- Store between 2 and 30ºC. Avoid temperatures above or below these values. When stored under these conditions, the contents of the kit have a shelf-life of minimum 12 months from date of production. The expiry date is shown on both tubes and cartons. The shelf-life described on the product label is only applicable when the product is stored according to manufacturer’s recommendations.
- No antibiotics, unstable additives or preservatives have been added to PureSperm SpeediKit.
Precautions and warnings
- When retrieving the sperm pellet, follow the instructions given in the pack insert to avoid inadvertent contamination.
- Use aseptic procedures at all times.
- If available, use sealed buckets during centrifugation to avoid creation of aerosols.
- Clean accidental spills using a dampened cloth or paper. Spilled PureSperm SpeediKit causes floors and benches to be extremely slippery.
- PureSperm SpeediKit does not present a fire or combustion hazard. A material safety data sheet is available from the distributor or manufacturer.
- Do not use any solution which shows evidence of bacterial contamination.
- Do not use contents if tamper-evident seal is broken.
- Federal Law (USA) restricts this device to sale by or on the order of a physician.
- Please check for regulatory compliance governing the use of ART products in your country.
General recommendations
N/A
Documents
References
FAQ
What do I need to perform the sperm preparation using Speedikit?
You only need a centrifuge and some sterile pipettes. PureSperm Speedikit includes the prefilled tubes for both separation and washing of the sample.
Do I need an incubator for performing a sperm preparation with Speedikit?
No, the whole procedure is performed at room temperature.